Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Thermo Scientific Chemicals BIS-TRIS propane, 0.2M buffer soln., pH 8.0
CAS: 64431-96-5 Molecular Formula: C11H26N2O6 Molecular Weight (g/mol): 282.337 MDL Number: MFCD00004689 InChI Key: HHKZCCWKTZRCCL-UHFFFAOYSA-N PubChem CID: 125132 ChEBI: CHEBI:40947 IUPAC Name: 2-[3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propylamino]-2-(hydroxymethyl)propane-1,3-diol SMILES: C(CNC(CO)(CO)CO)CNC(CO)(CO)CO
| PubChem CID | 125132 |
|---|---|
| CAS | 64431-96-5 |
| Molecular Weight (g/mol) | 282.337 |
| ChEBI | CHEBI:40947 |
| MDL Number | MFCD00004689 |
| SMILES | C(CNC(CO)(CO)CO)CNC(CO)(CO)CO |
| IUPAC Name | 2-[3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propylamino]-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | HHKZCCWKTZRCCL-UHFFFAOYSA-N |
| Molecular Formula | C11H26N2O6 |
Tris(hydroxymethyl)aminomethane 99.0+%, TCI America™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, Trimethylolaminomethane, Tris-Base PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | 2-Amino-2-(hydroxymethyl)-1,3-propanediol, Trimethylolaminomethane, Tris-Base |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
BES, MP Biomedicals™
CAS: 10191-18-1 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00007533 InChI Key: AJTVSSFTXWNIRG-UHFFFAOYSA-N Synonym: N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine PubChem CID: 73243 ChEBI: CHEBI:39045 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid SMILES: OCCN(CCO)CCS(O)(=O)=O
| PubChem CID | 73243 |
|---|---|
| CAS | 10191-18-1 |
| Molecular Weight (g/mol) | 213.25 |
| ChEBI | CHEBI:39045 |
| MDL Number | MFCD00007533 |
| SMILES | OCCN(CCO)CCS(O)(=O)=O |
| Synonym | N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine |
| IUPAC Name | 2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid |
| InChI Key | AJTVSSFTXWNIRG-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |
Gibco™ HBSS, no calcium, no magnesium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Includes | No Sodium Pyruvate |
|---|---|
| Physical Form | Liquid |
| pH | 6.7 to 7.8 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Hanks' Balanced Salt Solution (HBSS) |
| For Use With (Application) | Mammalian Cell Culture |
| Sterility | Sterile-filtered |
| Solution Type | Hank's Balanced Salt |
| Product Line | Gibco |
Corning™ Mediatech™ Cell Culture Phosphate Buffered Saline (10X)
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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With low endotoxin content
PIPES Sesquisodium Salt, MP Biomedicals™
CAS: 100037-69-2 Molecular Formula: C16H33N4Na3O12S4 InChI Key: FPDACHMSOMILQI-UHFFFAOYSA-K Synonym: 1, 4-Piperazinediethanesulfonic acid sesquisodium salt,Piperazine-1, 4-bis(2-ethanesulfonic acid) sesquisodium salt PubChem CID: 3086462
| PubChem CID | 3086462 |
|---|---|
| CAS | 100037-69-2 |
| Synonym | 1, 4-Piperazinediethanesulfonic acid sesquisodium salt,Piperazine-1, 4-bis(2-ethanesulfonic acid) sesquisodium salt |
| InChI Key | FPDACHMSOMILQI-UHFFFAOYSA-K |
| Molecular Formula | C16H33N4Na3O12S4 |
R&D Systems™ 10X PBS
R&D Systems™ 10X PBS (phosphate buffered saline) buffer is manufactured without magnesium or calcium, and is suitable for cell resuspension and washing in the in situ apoptosis detection (TUNEL) procedure.
MES, Monohydrate, cGMP Manufactured, 99.5%, Spectrum™ Chemical
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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CAS: 145224-94-8 Molecular Formula: C6H13NO4S Molecular Weight (g/mol): 213.25 InChI Key: SXGZJKUKBWWHRA-UHFFFAOYSA-N IUPAC Name: 4-(2-sulfonatoethyl)morpholin-4-ium SMILES: [O-]S(=O)(=O)CC[NH+]1CCOCC1
| CAS | 145224-94-8 |
|---|---|
| Molecular Weight (g/mol) | 213.25 |
| SMILES | [O-]S(=O)(=O)CC[NH+]1CCOCC1 |
| IUPAC Name | 4-(2-sulfonatoethyl)morpholin-4-ium |
| InChI Key | SXGZJKUKBWWHRA-UHFFFAOYSA-N |
| Molecular Formula | C6H13NO4S |
MilliporeSigma™ Calbiochem™ OmniPur™ PBS 10X Liquid Concentrate, Molecular Biology Grade, Sterile
Makes 10L of 1X PBS Buffer from each liter.
MilliporeSigma™ MOPS, Free Acid, Molecular Biology Grade, Calbiochem™,
CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 3-(N-Morpholino)propanesulfonic Acid PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 4-(3-sulfonatopropyl)morpholin-4-ium SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1
| PubChem CID | 70807 |
|---|---|
| CAS | 1132-61-2 |
| Molecular Weight (g/mol) | 209.26 |
| ChEBI | CHEBI:44115 |
| MDL Number | MFCD00006183 |
| SMILES | [O-]S(=O)(=O)CCC[NH+]1CCOCC1 |
| Synonym | 3-(N-Morpholino)propanesulfonic Acid |
| IUPAC Name | 4-(3-sulfonatopropyl)morpholin-4-ium |
| InChI Key | DVLFYONBTKHTER-UHFFFAOYSA-N |
| Molecular Formula | C7H15NO4S |
Gibco™ NaCl Solutions
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Gibco concentrates are intended for dilution at the point of use and come sterile-filtered and packaged in conveniently sized bioprocess containers. Sourcing concentrated stock solutions can help streamline buffer preparation by helping reduce preparation time, storage space, and the manufacturing footprint required for hydration.
| Percent Purity | Not Available |
|---|---|
| CAS | 7647-14-5 |
| For Use With (Equipment) | ILC/ILD System |
| Molecular Weight (g/mol) | Raw Material: 58.44 |
| Packaging | Bioprocess Container in Secondary Packaging |
| Physical Form | Solution |
| pH | 3.0 to 10.0 |
| Manufacturing Quality | CGMP |
| Chemical Name or Material | Raw Material: Sodium Chloride |
| Grade | EP/USP |
| For Use With (Application) | Bioproduction |
| Solution Type | Buffer |
MilliporeSigma™ TRIS Hydrochloride, OmniPur™, Calbiochem™,
CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: Trizma, Tris(hydroxymethyl)aminomethane PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
| PubChem CID | 93573 |
|---|---|
| CAS | 1185-53-1 |
| Molecular Weight (g/mol) | 157.594 |
| SMILES | C(C(CO)(CO)N)O.Cl |
| Synonym | Trizma, Tris(hydroxymethyl)aminomethane |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
| InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| Molecular Formula | C4H12ClNO3 |
MilliporeSigma™ MES, Sodium Salt, ULTROL™ Grade, Calbiochem™,
CAS: 71119-23-8 Molecular Formula: C6H12NNaO4S Molecular Weight (g/mol): 217.215 InChI Key: IRHWMYKYLWNHTL-UHFFFAOYSA-M Synonym: 2-(N-Morpholino)ethanesulfonic Acid, Na PubChem CID: 23673676 ChEBI: CHEBI:62955 IUPAC Name: sodium;2-morpholin-4-ylethanesulfonate SMILES: C1COCCN1CCS(=O)(=O)[O-].[Na+]
| PubChem CID | 23673676 |
|---|---|
| CAS | 71119-23-8 |
| Molecular Weight (g/mol) | 217.215 |
| ChEBI | CHEBI:62955 |
| SMILES | C1COCCN1CCS(=O)(=O)[O-].[Na+] |
| Synonym | 2-(N-Morpholino)ethanesulfonic Acid, Na |
| IUPAC Name | sodium;2-morpholin-4-ylethanesulfonate |
| InChI Key | IRHWMYKYLWNHTL-UHFFFAOYSA-M |
| Molecular Formula | C6H12NNaO4S |
MilliporeSigma™ MOPS, Sodium, ULTROL™ Grade, Calbiochem™,
CAS: 71119-22-7 Molecular Formula: C7H14NNaO4S Molecular Weight (g/mol): 231.242 InChI Key: MWEMXEWFLIDTSJ-UHFFFAOYSA-M Synonym: 3-(N-Morpholino)propanesulfonic Acid, Na PubChem CID: 3859613 IUPAC Name: sodium;3-morpholin-4-ylpropane-1-sulfonate SMILES: C1COCCN1CCCS(=O)(=O)[O-].[Na+]
| PubChem CID | 3859613 |
|---|---|
| CAS | 71119-22-7 |
| Molecular Weight (g/mol) | 231.242 |
| SMILES | C1COCCN1CCCS(=O)(=O)[O-].[Na+] |
| Synonym | 3-(N-Morpholino)propanesulfonic Acid, Na |
| IUPAC Name | sodium;3-morpholin-4-ylpropane-1-sulfonate |
| InChI Key | MWEMXEWFLIDTSJ-UHFFFAOYSA-M |
| Molecular Formula | C7H14NNaO4S |